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. 2006 Mar 25;49(2):8. doi: 10.1007/s11427-006-0158-7

Identification of one peptide which inhibited infectivity of avian infectious bronchitis virus in vitro

Bo Peng 1, Hanyang Chen 1, Yadi Tan 1,2, Meilin Jin 1,2, Huanchun Chen 1,2, Aizhen Guo 1,2,✉
PMCID: PMC7088975  PMID: 16704119

Abstract

Purified avian infectious bronchitis virus (IBV) was used to screen a random phage display peptide library. After the fourth panning, 10 positive phages were sequenced and characterized. The phages specifically inhibited IBV infectivity in HeLa cells and blocked IBV haemagglutination. One linear peptide “GSH HRH VHS PFV” from the positive phages with the highest neutralization titer was synthesized and this peptide inhibited IBV infection in HeLa as well. The results may contribute to development of antiviral therapeutics for IBV and studying the determinants for viral and cell interaction.

Keywords: avian infectious bronchitis virus, phage display, peptides

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