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. 1995;19(5):375–407. doi: 10.1007/BF01839319

Applications of DNA amplification techniques in veterinary diagnostics

M Pfeffer 1,✉, M Wiedmann 2, C A Batt 2
PMCID: PMC7089009  PMID: 8560754

Abstract

An overview of the principles of the polymerase chain reaction, ligase chain reaction, self-sustained sequence replication and Qβ replicase is given. The application of these methods for the diagnosis of veterinary infectious and hereditary diseases as well as for other diagnostic purposes is discussed and comprehensive tables of reported assays are provided. Specific areas where these DNA-based amplification methods provide substantial advantages over traditional approaches are also highlighted. With regard to PCR-based assays for the detection of viral pathogens, this article is an update of a previous review by Belák and Ballagi-Pordány (1993).

Keywords: diagnosis, LCR, ligase chain reaction, PCR, polymerase chain reaction, Qβ replicase, self-sustained sequence replication, 3SR

Abbreviations

3SR

self-sustained sequence replication

BHV

bovine herpesvirus

BLAD

bovine leukocyte adhesion deficiency

cDNA

complementary DNA

CFU

colony-forming units

EHV

equine herpesvirus

ELISA

enzyme-linked immunosorbent assay

FHV

feline herpesvirus

LCR

ligase chain reaction

PCR

polymerase chain reaction

PRV

pseudorabies virus

RAPD

random amplified polymorphic DNA

RFLP

restriction fragment length polymorphism

RT

reverse transcriptase

rRNA

ribosomal RNA

wt

wild-type

Footnotes

These two authors contributed equally to this review and are listed in alphabetical order.

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