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. 2016 Oct 17;59(11):1166–1176. doi: 10.1007/s11427-016-0202-8

Differential expression analysis of the broiler tracheal proteins responsible for the immune response and muscle contraction induced by high concentration of ammonia using iTRAQ-coupled 2D LC-MS/MS

Yan Xiong 1, Xiangfang Tang 1, Qingshi Meng 1, Hongfu Zhang 1,✉
PMCID: PMC7089013  PMID: 27761697

Abstract

Ammonia has been considered the contaminant primarily responsible for respiratory disease in poultry. Even though it can cause tracheal lesions, its adverse effects on the trachea have not been sufficiently studied. The present study investigated tracheal changes in Arbor Acres broilers (Gallus gallus) induced by high concentration of ammonia using isobaric tag for relative and absolute quantification (iTRAQ)-based proteome analysis. In total, 3,706 proteins within false discovery rate of 1% were identified, including 119 significantly differentially expressed proteins. Functional analysis revealed that proteins related to immune response and muscle contraction were significantly enriched. With respect to the immune response, up-regulated proteins (like FGA) were pro-inflammatory, while down-regulated proteins participated in antigen processing and antigen presenting (like MYO1G), immunoglobulin and cathelicidin production (like fowlicidin-2), and immunodeficiency (like PTPRC). Regarding muscle contraction, all differentially expressed proteins (like TPM1) were up-regulated. An over-expression of mucin, which is a common feature of airway disease, was also observed. Additionally, the transcriptional alterations of 6 selected proteins were analyzed by quantitative RT-PCR. Overall, proteomic changes suggested the onset of airway obstruction and diminished host defense in trachea after ammonia exposure. These results may serve as a valuable reference for future interventions against ammonia toxicity.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s11427-016-0202-8 and is accessible for authorized users.

Keywords: ammonia, broiler, proteomics, trachea

Electronic supplementary material

11427_2016_202_MOESM1_ESM.xls (647KB, xls)

Table S1, Description of the 3706 identified proteins in the broiler tracheal sample

11427_2016_202_MOESM2_ESM.xls (85.5KB, xls)

Table S2, The list of the 119 differential expressed proteins

11427_2016_202_MOESM3_ESM.xls (70KB, xls)

Table S3, The primers used for the amplification of target genes

Footnotes

This article is published with open access at link.springer.com

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

11427_2016_202_MOESM1_ESM.xls (647KB, xls)

Table S1, Description of the 3706 identified proteins in the broiler tracheal sample

11427_2016_202_MOESM2_ESM.xls (85.5KB, xls)

Table S2, The list of the 119 differential expressed proteins

11427_2016_202_MOESM3_ESM.xls (70KB, xls)

Table S3, The primers used for the amplification of target genes

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