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. 2007;54(6):770–775. doi: 10.1134/S1021443707060088

Tissue specific expression of hepatitis B virus surface antigen in transgenic plant cells and tissue culture

E B Rukavtsova 1,✉, T V Abramikhina 1, N Ya Shulga 2, V A Bykov 2, Ya I Bur’yanov 1
PMCID: PMC7089057  PMID: 32214751

Abstract

The tobacco plants (Nicotiana tabacum L.) carrying the HBsAg gene controlled by (Aocs)3AmasPmas, the hybrid promoter that includes regulatory elements of the agrobacterial octopine and mannopine synthase genes, as well as plants controlled by the same promoter and adh1, maize alcohol dehydrogenase gene intron were obtained. The presence of the adh1 gene intron did not significantly change the level of expression of the HBsAg gene in plants. The analysis of expression of hepatitis B virus surface antigen (HBs-antigen) in transformed plants expressing the HBsAg under the control of different promoters was made. The level of HBs-antigen in plants carrying the HBsAg gene controlled by (Aocs)3AmasPmas, the hybrid agrobacterium-derived promoter, was the highest in roots and made up to 0.01% of total amount of soluble protein. The level of HBs-antigen in plants carrying the HBsAg gene controlled by the dual 35S RNA cauliflower mosaic virus promoter was the same in all organs of the plant and made up to 0.06% of the total amount of soluble protein. Hairy root and callus cultures of plants carrying the HBsAg gene and expressing the HBs-antigen were obtained.

Key words: Nicotiana tabacum, transformation, promoters, HBsAg

Abbreviations

BA

benzyladenine

CaMV 35SS

dual 35S RNA cauliflower mosaic virus promoter

HAsAg, HBs-antigen

hepatitis B virus surface antigen

Km

kanamycin

MS

Murashige and Skoog nutrient medium

Footnotes

Original Russian Text © E.B. Rukavtsova, T.V. Abramikhina, N.Ya. Shulga, V.A. Bykov, Ya.I. Bur’yanov, 2007, published in Fiziologiya Rastenii, 2007, Vol. 54, No. 6, pp. 864–869.

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