Abstract
In an investigation of blood-sucking insects and arboviruses, a virus (YN12243) was isolated from Culicoides samples collected in the Sino-Burmese border region of Yunnan Province, China. The virus caused cytopathic effect (CPE) in C6/36 cells and passaged stably. Polyacrylamide gel analysis showed that the genome of YN12243 was composed of 12 segments of double-stranded RNA (dsRNA), with a distribution pattern of 6-6. The nucleotide and amino acid sequences of the coding region (1‒12 segments) were 17,803 bp and 5,925 amino acids in length, respectively. The phylogenetic analysis of VP1 protein (RdRp) revealed that YN12243 belonged to genus Seadornavirus of family Reoviridae, and further analysis indicated that YN12243 belongs to the Banna virus (BAV) genotype A2. Additionally, YN12243 was located in the same evolutionary cluster as BAV strains isolated from different mosquito species, suggesting that the BAV isolated from Culicoides does not have species barriers. These results indicate that Culicoides can also be a vector for BAV. In view of the hematophagous habits of Culicoides on cattle, horses, deer, and other large animals, as well as the possibility of spreading and causing a variety of animal arboviral diseases, it is important to improve infection detection and monitor the BAV in large livestock.
Keywords: Banna virus, Seadornavirus, Reoviridae, mosquito, Culicoides
Acknowledgements
This work was supported by the National Natural Science Foundation of China (81290342, 81501757), the Special National Project on Research and Development of Key Biosafety Technologies (2016YFC1201904), the National Key Plan for Scientific Research and Development of China (2016YFD0500300) and the development grants of State Key Laboratory of Infectious Disease Prevention and Control (2014 SKLID103, 2015SKLID505).
Footnotes
Contributed equally to this work
Contributor Information
Xiaoqing Lu, Email: Luxq532@126.com.
Guodong Liang, Email: gdliang@hotmail.com.
References
- Attoui H., Billoir F., Biagini P., de Micco P., de Lamballerie X. Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae. J Gen Virol. 2000;81:1507–1515. doi: 10.1099/0022-1317-81-6-1507. [DOI] [PubMed] [Google Scholar]
- Attoui H., Mohd Jaafar F., de Micco P., de Lamballerie X. Coltiviruses and Seadornaviruses in North America, Europe, and Asia. Emerg Infect Dis. 2005;11:1673–1679. doi: 10.3201/eid1111.050868. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Attoui H., Mohd Jaafar F., de Lamballerie X., Mertens P.P. Seadornavirus, Reoviridae. In: Fauquet C.M., Mayo M.A., Maniloff J., Desselberger U., Ball L.A., editors. Eighth Report of the International Committee on Taxonomy of Viruses. London: Elsevier Academic Press; 2005. pp. 504–510. [Google Scholar]
- Brown S.E., Gorman B.M., Tesh R.B., Knudson D.L. Coltiviruses isolated from mosquitoes collected in Indonesia. Virology. 1993;196:363–367. doi: 10.1006/viro.1993.1490. [DOI] [PubMed] [Google Scholar]
- Kim H.K., Yoon S.W., Kim D.J., Koo B.S., Noh J.Y., Kim J.H., Choi Y.G., Na W., Chang K.T., Song D., Jeong D.G. Detection of severe acute respiratory syndrome-like, middle east respiratory syndrome-like bat coronaviruses and group H rotavirus in faeces of Korean Bats. Transbound Emerg Dis. 2016;63:365–372. doi: 10.1111/tbed.12515. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deng Y.Q., Zhao H., Li X.F., Zhang N.N., Liu Z.Y., Jiang T., Gu D.Y., Shi L., He J.A., Wang H.J., Sun Z.Z., Ye Q., Xie D.Y., Cao W.C., Qin C.F. Isolation, identification and genomic characterization of the Asian lineage Zika virus imported to China. Sci China Life Sci. 2016;59:428–430. doi: 10.1007/s11427-016-5043-4. [DOI] [PubMed] [Google Scholar]
- Kou M., Zhu J., Yang H., Xiao L., Wang J., Miao H., Wang J., Li H. First isolation and idengtification of Banna virus from Culicoides pools in Yunnan (in Chinese) Chin J Exp Clin Virol. 2016;30:1–4. [Google Scholar]
- Li Z., Qi X.L., Ren X.G., Cui L., Wang X.M., Zhu P. Molecular characteristics and evolutionary analysis of a very virulent infectious bursal disease virus. Sci China Life Sci. 2015;58:731–738. doi: 10.1007/s11427-015-4900-x. [DOI] [PubMed] [Google Scholar]
- Liu H., Liang G. Banna virus and its infection (in Chinese) Chin J Vector Biol Control. 2010;21:500–502. [Google Scholar]
- Liu H., Li M.H., Zhai Y.G., Meng W.S., Sun X.H., Cao Y.X., Fu S.H., Wang H.Y., Xu L.H., Tang Q., Liang G.D. Banna virus, China, 1987–2007. Emerg Infect Dis. 2010;16:514–517. doi: 10.3201/eid1603.091160. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liu H., Gao X., Liang G. Newly recognized mosquito-associated viruses in mainland China, in the last two decades. Virol J. 2011;8:68. doi: 10.1186/1743-422X-8-68. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liu H., Liang G.D. The genotype A specific primers for amplifying and sequencing the whole genome of Banna virus (in Chinese) Chin J Vector Biol Control. 2016;27:533–538. [Google Scholar]
- Liu H., Gao X.Y., Fu S.H., Li M.H., Zhai Y.G., Meng W.S., Sun X.H., Lv Z., Wang H.Y., Shen X.X., Cao Y.X., He Y., Liang G.D. Molecular evolution of emerging Banna virus. Infect Genet Evol. 2016;45:250–255. doi: 10.1016/j.meegid.2016.08.034. [DOI] [PubMed] [Google Scholar]
- Lu S., Zhang D., Zhou J., Xia Z., Lin D., Lou Y., Tan W., Qin K. Prevalence and phylogenetic characterization of canine coronavirus from diseased pet dogs in Beijing, China. Sci China Life Sci. 2016;59:860–862. doi: 10.1007/s11427-015-0369-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mohd Jaafar F., Attoui H., Mertens P.P.C., de Micco P., de Lamballerie X. Structural organization of an encephalitic human isolate of Banna virus (genus Seadornavirus, family Reoviridae) J General Virol. 2005;86:1147–1157. doi: 10.1099/vir.0.80578-0. [DOI] [PubMed] [Google Scholar]
- Nabeshima T., Thi Nga P., Guillermo P., Parquet M.C., Yu F., Thanh Thuy N., Minh Trang B., Tran Hien N., Sinh Nam V., Inoue S., Hasebe F., Morita K. Isolation and molecular characterization of Banna virus from mosquitoes, Vietnam. Emerg Infect Dis. 2008;14:1276–1279. doi: 10.3201/eid1408.080100. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reuter G., Boros A., Delwart E., Pankovics P. Novel Seadornavirus (family Reoviridae) related to Banna virus in Europe. Arch Virol. 2013;158:2163–2167. doi: 10.1007/s00705-013-1712-9. [DOI] [PubMed] [Google Scholar]
- Shi M., Lin X.D., Tian J.H., Chen L.J., Chen X., Li C.X., Qin X.C., Li J., Cao J.P., Eden J.S., Buchmann J., Wang W., Xu J., Holmes E.C., Zhang Y.Z. Redefining the invertebrate RNA virosphere. Nature. 2016;540:539–543. doi: 10.1038/nature20167. [DOI] [PubMed] [Google Scholar]
- Sun X., Fu S., Gong Z., Ge J., Meng W., Feng Y., Wang J., Zhai Y., Wang H., Nasci R., Wang H., Tang Q., Liang G. Distribution of arboviruses and mosquitoes in northwestern Yunnan Province, China. Vector-Borne Zoonotic Dis. 2009;9:623–630. doi: 10.1089/vbz.2008.0145. [DOI] [PubMed] [Google Scholar]
- Tao S.J., Chen B.Q. Studies of Coltivirus in China. Chin Med J (Engl) 2005;118:581–586. [PubMed] [Google Scholar]
- Wang J., Zhang H., Sun X., Fu S., Wang H., Feng Y., Wang H., Tang Q., Liang G.D. Distribution of mosquitoes and mosquito-borne arboviruses in Yunnan Province near the China-Myanmar-Laos border. Am J Trop Med Hyg. 2011;84:738–746. doi: 10.4269/ajtmh.2011.10-0294. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang J., Li H., He Y., Zhou Y., Meng J., Zhu W., Chen H., Liao D., Man Y. Isolation and genetic characterization of Mangshi virus: a newly discovered Seadornavirus of the Reoviridae family found in Yunnan Province, China. PLoS ONE. 2015;10:e0143601. doi: 10.1371/journal.pone.0143601. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Xu P., Wang Y., Zuo J., Lin J., Xu P. New orbiviruses isolated from patients with unknown fever and encephalitis in Yunnan province (in Chinese) Chin J Virol. 1990;6:27–33. [Google Scholar]
- Xu P., Wang Y., Zuo J., Che Y., Peng H., Huang Z., Tang G., Xu P. Recovery of the same type of virus as human new orbivirus (in Chinese) Chin J Virol. 1990;6:328–332. [Google Scholar]
- Yang J.M., Yin G.Q., Feng Y.H., Luo Z.Y., Jiao J.F. Co-infection of Coltivirus and Japanese encephalitis virus. J Nanjing Railway Med College. 1996;15:169. [Google Scholar]