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. 1986;72(1):47–65. doi: 10.1007/BF00230635

Modifications of lysosomal enzymes in Dictyostelium discoideum

Hudson H Freeze 1,✉
PMCID: PMC7089276  PMID: 2434832

Abstract

This paper has two purposes. The first is to review the past studies on the structure, biosynthesis, and immunological properties of a class of glycoproteins, the lysosomal enzymes, in Dictyostelium discoideum. The second purpose is to present new data on the analysis of mutant strains altered in the biosynthesis of the lipid-linked precursor of N-linked oligosaccharides, and on the characterization of new carbohydrate antigenic determinants found on multiple proteins in Dictyostelium. We will also show how a combination of genetic, biochemical and immunochemical approaches have been used to unravel a portion of the glycosylation pathway in Dictyostelium.

The long-term goal of these studies is to use Dictyostelium discoideum as a model system to understand the functions of a variety of glycoconjugates in a multicellular organism. The existence of a large number of mutant strains which are altered in a variety of cellular functions, development and the posttranslational modification of multiple proteins, offers a great opportunity to explore this area.

Keywords: Dictyostelium discoideum, lysosomal enzymes, glycosylation, sulfated N-linked oligosaccharides, common antigens

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