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. 2007;52(20):2805–2810. doi: 10.1007/s11434-007-0436-1

Immune response in cattle inoculated with the recombinant complete polyprotein of foot-and-mouth disease virus from Bombyx mori larvae

ZhiYong Li 1, YongZhu Yi 2, XiangPing Yin 1, ZhiFang Zhang 2,✉, JiXing Liu 1,✉
PMCID: PMC7089379  PMID: 32214727

Abstract

The intact open reading frame (ORF) of foot-and-mouth disease virus (FMDV) Asia I/XJ strain was amplified by RT-PCR and inserted into the transfer vector pVL1393 to generate plasmid pVL-ORF. Bm-N cells were transfected with pVL-ORF and linearized Bm-BacPAK6 DNA, and the recombinant silkworm baculovirus Bm-ORF containing the full ORF of FMDV was obtained. The results of indirect immunofluorescence assay (IFA) showed that Bm-ORF could be expressed efficiently in Bm-N cell. After inoculating the early 5th instar larvae of silkworm, the polyprotein of FMDV could be detected by sandwich ELISA and empty capsid-like particles could be observed under the electron microscope. Expression products from silkworm were used as the antigen to immunize the cattle. The specific antibody was induced in all vaccinated animals. The immunized cattle were challenged with the virulent FMDV Asia I/XJ strain, two of the four cattle were completely protected and clinical symptoms were alleviated and delayed in the others. The results suggest that this strategy might be used to develop the new subunit FMDV vaccine.

Keywords: foot-and-mouth disease virus, open reading frame, silkworm-baculovirus expression system, immunogenicity, subunit vaccine

Footnotes

Supported by the National 863 Project of China (Grant No. 2006AA02Z440) and Chinese Funding for Social Public Interests (Grant No. 2005DIB4J041)

Contributor Information

ZhiFang Zhang, Email: zhangzf@caas.net.cn.

JiXing Liu, Email: liujixing@hotmail.com.

References

  • 1.Mason P. W., Grubman M. J., Baxt B. Molecular basis of pathogenesis of FMDV. Virus Res. 2003;91(1):9–32. doi: 10.1016/S0168-1702(02)00257-5. [DOI] [PubMed] [Google Scholar]
  • 2.Beck E., Strohmaier K. Subtyping of European foot-and-mouth disease virus strains by nucleotide sequence determination. J Virol. 1987;61:1621–1629. doi: 10.1128/jvi.61.5.1621-1629.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Rweyemamu M. M., Terry G., Pay T. W. Stability and immunogenicity of empty particles of foot-and-mouth disease virus. Arch Virol. 1979;59:69–79. doi: 10.1007/BF01317896. [DOI] [PubMed] [Google Scholar]
  • 4.Rowlands D. J., Sangar D. V., Brown F. A comparative chemical and serological study of the full and empty particles of foot-and-mouth disease virus. J Gen Virol. 1975;26:227–238. doi: 10.1099/0022-1317-26-3-227. [DOI] [PubMed] [Google Scholar]
  • 5.Noad R., Roy P. Virus-like particles as immunogens. Trends Microbiol. 2003;11(9):438–444. doi: 10.1016/S0966-842X(03)00208-7. [DOI] [PubMed] [Google Scholar]
  • 6.Lin X. A., Zhang W., Chen Y., et al. Overexpression of celB gene coding for β-Glucosidase from Pyrococcus furiosus using baculovirus expression vector system in silkworm, Bombyx mori. Z Natforsch. 2006;61:595–600. doi: 10.1515/znc-2006-7-821. [DOI] [PubMed] [Google Scholar]
  • 7.Choi J. Y., Woo S. D., Lee H. K., et al. High-level expression of canine parvovirus VP2 using Bombyx mori nucleopolyhedrovirus vector. Arch Virol. 2000;145(1):171–177. doi: 10.1007/s007050050014. [DOI] [PubMed] [Google Scholar]
  • 8.Mayr G. A., O’Donnell V., Chinsangaram J., et al. Immune responses and protection against foot-and-mouth disease virus (FMDV) challenge in swine vaccinated with adenovirus-FMDV constructs. Vaccine. 2001;19:2152–2162. doi: 10.1016/S0264-410X(00)00384-4. [DOI] [PubMed] [Google Scholar]
  • 9.Moraes M. P., Chinsangaram J., Brum M. C. S., et al. Immediate protection of swine from foot-and-mouth disease: A combination of adenoviruses expressing interferon alpha and a foot-and-mouth disease virus subunit vaccine. Vaccine. 2003;22:268–279. doi: 10.1016/S0264-410X(03)00560-7. [DOI] [PubMed] [Google Scholar]
  • 10.Pacheco J. M., Brum M. C. S., Moraes M. P., et al. Rapid protection of cattle from direct challenge with foot-and-mouth disease virus (FMDV) by a single inoculation with an adenovirus-vectored FMDV subunit vaccine. Virology. 2005;337:205–209. doi: 10.1016/j.virol.2005.04.014. [DOI] [PubMed] [Google Scholar]
  • 11.Moraes M. P., Mayr G. A., Mason P. W., et al. Early protection against homologous challenge after a single dose of replication-defective human adenovirus type 5 expressing capsid proteins of foot-and-mouth disease virus (FMDV) strain A24. Vaccine. 2002;20:1631–1639. doi: 10.1016/S0264-410X(01)00483-2. [DOI] [PubMed] [Google Scholar]
  • 12.Guo H. C., Liu Z. X., Sun S. Q., et al. Immune response in guinea pigs vaccinated with DNA vaccine of foot-and-mouth disease virus O/China99. Vaccine. 2005;23:3236–3242. doi: 10.1016/j.vaccine.2004.03.074. [DOI] [PubMed] [Google Scholar]
  • 13.Dus Santos M. J., Carrillo C., Ardila F. Development of transgenic alfalfa plants containing the foot and mouth disease virus structural polyprotein gene P1 and its utilization as an experimental immunogen. Vaccine. 2005;23:1838–1843. doi: 10.1016/j.vaccine.2004.11.014. [DOI] [PubMed] [Google Scholar]
  • 14.Wu X F, Chinese patent, CN1242428
  • 15.Zheng H. X., Liu X. T., Shang Y. J., et al. Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain. Chin Sci Bull. 2006;51(17):2072–2078. doi: 10.1007/s11434-006-2095-z. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Brown F. The history of research in foot-and-mouth disease. Virus Res. 2003;91:3–7. doi: 10.1016/S0168-1702(02)00268-X. [DOI] [PubMed] [Google Scholar]
  • 17.Guo H., Liu X., Liu Z. Recent outbreaks of foot-and-mouth disease type Asia 1 in China. J Vet Med B. 2006;53:29–33. doi: 10.1111/j.1439-0450.2006.01018.x. [DOI] [Google Scholar]
  • 18.Zhang X. W., Wang Q., Liu J. X., et al. Construction and identification of recombinant adenovirus containing the polyproteins coding regions of O type foot-and-mouth disease virus by homologous recombination in Escherichia coli. Acta Microbiol Sin (in Chinese) 2006;46(2):223–226. [PubMed] [Google Scholar]
  • 19.Lü H. S. Molecular Biology of Insect Viruses (in Chinese) Beijing: China Agricultural Scientech Press; 1998. p. 547. [Google Scholar]
  • 20.Rueda P., Fominaya J., Langeveld J. P. M., et al. Effect of different baculovirus inactivation procedures on the integrity and immunogenicity of porcine parvovirus-like particles. Vaccine. 2000;19:726–734. doi: 10.1016/S0264-410X(00)00259-0. [DOI] [PubMed] [Google Scholar]
  • 21.Maranga L., Cruz P. E., Aunins J. G., et al. Production of core and virus-like particles with baculovirus infected insect cells. Adv Biochem Eng Biotechnol. 2002;74:183–206. doi: 10.1007/3-540-45736-4_9. [DOI] [PubMed] [Google Scholar]
  • 22.Mortola E., Roy P. Efficient assembly and release of SARS coronavirus-like particles by a heterologous expression system. FEBS Lett. 2004;576(1–2):174–178. doi: 10.1016/j.febslet.2004.09.009. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Chinese Science Bulletin = Kexue Tongbao are provided here courtesy of Nature Publishing Group

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