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. 2010 May 20;53(4):685–694. doi: 10.1007/s11426-010-0135-7

Recent advances in proteolysis and peptide/protein separation by chromatographic strategies

XiangMin Zhang 1, BaoHong Liu 1, LiHua Zhang 2, HanFa Zou 2, Jing Cao 1, MingXia Gao 1, Jia Tang 1, Yun Liu 1, PengYuan Yang 1,✉, YuKui Zhang 2,✉
PMCID: PMC7089403  PMID: 32214996

Abstract

This review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chromatographic strategies in the past ten years, covering the main research in these areas especially in China. The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. These advances have significantly promoted the research of protein/peptide separation and identification in proteomics.

Keywords: proteolysis, enzymatic digestion, chromatographic separation, peptide enrichment, mass spectrometry

Footnotes

Support from the Major State Basie Research Development Program (Grant No. 2007CB914100), the National Natural Science Foundation of China (Grant Nos. 20875016 & 20735005), and Shanghai Projects (Grant Nos. 08DZ2293601 & B109).

Contributor Information

PengYuan Yang, Email: pyyang@fudan.edu.cn.

YuKui Zhang, Email: ykzhang@dicp.ac.cn.

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