The content is available as a PDF (307.9 KB).
Electronic supplementary material
Acknowledgements
We thank Dr. Bart L. Haagmans and Dr. Ron A.M. Fouchier (Erasmus MC, Rotterdam, the Netherlands) for providing MERSCoV (isolate hCoV-EMC/2012). This work was supported by the National Key Research and Development Program of China (2016YFD0500300 to Wenjie Tan). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Footnotes
Contributed equally to this work
Contributor Information
Yusen Zhou, Email: yszhou@bmi.ac.cn.
Wenjie Tan, Email: tanwj28@163.com.
References
- Agrawal A.S., Ying T., Tao X., Garron T., Algaissi A., Wang Y., Wang L., Peng B.H., Jiang S., Dimitrov D.S., et al. Passive transfer of A germline-like neutralizing human monoclonal antibody protects transgenic mice against lethal middle east respiratory syndrome coronavirus infection. Sci Rep. 2016;6:31629. doi: 10.1038/srep31629. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Qiu H., Sun S., Xiao H., Feng J., Guo Y., Tai W., Wang Y., Du L., Zhao G., Zhou Y. Single-dose treatment with a humanized neutralizing antibody affords full protection of a human transgenic mouse model from lethal Middle East respiratory syndrome (MERS)-coronavirus infection. Antiviral Res. 2016;132:141–148. doi: 10.1016/j.antiviral.2016.06.003. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shi W., Li J., Zhou H., Gao G.F. Pathogen genomic surveillance elucidates the origins, transmission and evolution of emerging viral agents in China. Sci China Life Sci. 2017;60:1317–1330. doi: 10.1007/s11427-017-9211-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith K., Garman L., Wrammert J., Zheng N.Y., Capra J.D., Ahmed R., Wilson P.C. Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen. Nat Protoc. 2009;4:372–384. doi: 10.1038/nprot.2009.3. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ying T., Du L., Ju T.W., Prabakaran P., Lau C.C.Y., Lu L., Liu Q., Wang L., Feng Y., Wang Y., et al. Exceptionally potent neutralization of Middle East respiratory syndrome coronavirus by human monoclonal antibodies. J Virol. 2014;88:7796–7805. doi: 10.1128/JVI.00912-14. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zaki A.M. v, Boheemen S., Bestebroer T.M., Osterhaus A.D.M.E., Fouchier R.A.M. Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med. 2012;367:1814–1820. doi: 10.1056/NEJMoa1211721. [DOI] [PubMed] [Google Scholar]
- Zhao G., Du L., Ma C., Li Y., Li L., Poon V.K., Wang L., Yu F., Zheng B.J., Jiang S., et al. A safe and convenient pseudovirus-based inhibition assay to detect neutralizing antibodies and screen for viral entry inhibitors against the novel human coronavirus MERS-CoV. Virol J. 2013;10:266. doi: 10.1186/1743-422X-10-266. [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.