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. 2014 Mar 7;52(5):435–440. doi: 10.1007/s12275-014-3529-y

Comparison of JEV neutralization assay using pseudotyped JEV with the conventional plaque-reduction neutralization test

Hee-Jung Lee 1, Kyung-Il Min 2, Ki Hoon Park 1, Hyo Jung Choi 1, Min-Kyoung Kim 1, Chi-Young Ahn 3, Young-Jin Hong 4, Young Bong Kim 1,✉
PMCID: PMC7090846  PMID: 24610332

Abstract

We previously reported the development of a neutralization assay system for evaluating Japanese Encephalitis Virus (JEV) neutralizing antibody (NAb) using pseudotyped-JEV (JEV-PV). JEV-PV-based neutralization assay offers several advantages compared with the current standard plaque-reduction neutralization test (PRNT), including simplicity, safety, and speed. To evaluate the suitability of the JEV-PV assay as new replacement neutralization assay, we compared its repeatability, reproducibility, specificity, and correlated its results with those obtained using the PRNT. These analyses showed a close correlation between the results obtained with the JEV-PV assay and the PRNT, using the 50% plaque reduction method as a standard for measuring NAb titers to JEV. The validation results met all analytical acceptance criteria. These results suggest that the JEV-PV assay could serve as a safe and simple method for measuring NAb titer against JEV and could be used as an alternative approach for assaying the potency of JEV neutralization.

Keywords: Japanese encephalitis virus, neutralizing antibody, pseudovirus, PRNT

Footnotes

These authors contributed equally to this work Copyright 2014, The Microbiological Society of Korea

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