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. 2018 Apr 2;56(4):272–279. doi: 10.1007/s12275-018-7504-x

A murine colitis model developed using a combination of dextran sulfate sodium and Citrobacter rodentium

Jin-Il Park 1,2, Sun-Min Seo 1, Jong-Hyung Park 1, Hee-Yeon Jeon 1,3, Jun-Young Kim 1, Seung-Hyun Ryu 1,4, Yang-Kyu Choi 1,✉
PMCID: PMC7090851  PMID: 29611140

Abstract

Adult mice were treated with dextran sulfate sodium (DSS) and infected with Citrobacter rodentium for developing a novel murine colitis model. C57BL/6N mice (7-week-old) were divided into four groups. Each group composed of control, dextran sodium sulfate-treated (DSS), C. rodentium-infected (CT), and DSS-treated and C. rodentium-infected (DSS-CT) mice. The DSS group was administered 1% DSS in drinking water for 7 days. The CT group was supplied with normal drinking water for 7 days and subsequently infected with C. rodentium via oral gavage. The DSS-CT group was supplied with 1% DSS in drinking water for 7 days and subsequently infected with C. rodentium via oral gavage. The mice were sacrificed 10 days after the induction of C. rodentium infection. The DSS-CT group displayed significantly shorter colon length, higher spleen to body weight ratio, and higher histopathological score compared to the other three groups. The mRNA expression levels of tumor necrosis factor (TNF)-α and interferon (INF)-γ were significantly upregulated; however, those of interleukin (IL)-6 and IL-10 were significantly downregulated in the DSS-CT group than in the control group. These results demonstrated that a combination of low DSS concentration (1%) and C. rodentium infection could effectively induce inflammatory bowel disease (IBD) in mice. This may potentially be used as a novel IBD model, in which colitis is induced in mice by the combination of a chemical and a pathogen.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s12275-018-7504-x and is accessible for authorized users.

Keywords: Citrobacter rodentium, colitis, dextran sulfate sodium

Electronic supplementary material

12275_2018_7504_MOESM1_ESM.pdf (875.4KB, pdf)

Supplementary material, approximately 875 KB.

Footnotes

A correction to this article is available at 10.1007/s12275-018-0578-7

Change history

5/2/2018

In the article by Park <Emphasis Type="Italic">et al.</Emphasis> published in Journal of Microbiology 2018; 56, 272–279, the supplementary data Figs S1 and S2 should be corrected as below. The original article can be found online at <ExternalRef><RefSource>https://doi.org/10.1007/s12275-018-7504-x</RefSource><RefTarget Address="https://doi.org/10.1007/s12275-018-7504-x" TargetType="URL"/></ExternalRef>.

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Supplementary Materials

12275_2018_7504_MOESM1_ESM.pdf (875.4KB, pdf)

Supplementary material, approximately 875 KB.

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