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. 2011 Jan 9;48(6):849–855. doi: 10.1007/s12275-010-0132-8

Multilocus sequence typing and virulence factors analysis of Escherichia coli O157 strains in China

Xiao W Ji 1, Ya L Liao 1, Ye F Zhu 2, Hai G Wang 1, Ling Gu 2, Jiang Gu 1, Chen Dong 2, Hong L Ding 1, Xu H Mao 1, Feng C Zhu 2,✉, Quan M Zou 1,✉
PMCID: PMC7091087  PMID: 21221945

Abstract

Escherichia coli O157:H7, an important food-borne pathogen, has become a major public health concern worldwide. The aim of this study was to investigate the molecular epidemiologic feature of E. coli O157:H7 strains in China. 105 E. coli O157:H7 isolates were collected from various hosts and places over 9 years. A multilocus sequence typing scheme (MLST) was applied for bacteria genotyping and polymerase chain reaction (PCR) was used for virulence factor identification. Seven new MLST sequence types (STs), namely ST836, ST837, ST838, ST839, ST840, ST841, and ST842 were identified, which grouped into two lineages. Phylogenetic analysis suggested that the most two frequent STs in China, ST837 and ST836, may be the derivatives of E. coli O157:H7 Sakai or E. coli O157:H7 EDL933. Geographical diversity and host variety of E. coli O157:H7 were observed in China. In addition, the different distribution of tccp was detected. The data presented herein provide new insights into the molecular epidemiologic feature of E. coli O157:H7, and aid in the investigation of the transmission regularity and evolutionary mechanism of E. coli O157:H7.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s12275-010-0132-8 and is accessible for authorized users.

Keywords: E. coli O157: H7, tccp, STs, stx

Electronic supplementary material

12275_2010_132_MOESM1_ESM.pdf (29.6KB, pdf)

Supplementary material, approximately 29.5 KB.

Footnotes

These authors contributed equally to this work.

Supplemental material for this article may be found at http://www.springer.com/content/120956

Contributor Information

Feng C. Zhu, Phone: +86-23-6875-2316, FAX: +86-23-6875-3591, Email: jszfc@vip.sina.com

Quan M. Zou, Email: qmzou@mail.tmmu.com.cn

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