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. 2016 Mar 18;21(1):128–133. doi: 10.1007/s12257-015-0384-6

A rapidly new-typed detection of norovirus based on F0F1-ATPase molecular motor biosensor

Zhuo Zhao 1, Jie Zhang 2,✉, Mei-Ling Xu 3, Zhi-Peng Liu 1, Hua Wang 1, Ming Liu 1, Yan-Yan Yu 1, Li Sun 1, Hui Zhang 1, Hai-Yan Wu 4
PMCID: PMC7091097  PMID: 32218681

Abstract

In order to adapt port rapid detection of food borne norovirus, presently we developed a new typed detection method based on F0F1-ATPase molecular motor biosensor. A specific probe was encompassed the conservative region of norovirus and F0F1-ATPase within chromatophore was constructed as a molecular motor biosensor through the “ε-subunit antibody-streptomycin-biotin-probe” system. Norovirus was captured based on probe-RNA specific binding. Our results demonstrated that the Limit of Quantification (LOQ) is 0.005 ng/mL for NV RNA and also demonstrated that this method possesses specificity and none cross-reaction for food borne virus. What’s more, the experiment used this method could be accomplished in 1 h. We detected 10 samples by using this method and the results were consistent with RT-PCR results. Overall, based on F0F1-ATPase molecular motors biosensor system we firstly established a new typed detection method for norovirus detection and demonstrated that this method is sensitive and specific and can be used in the rapid detection for food borne virus.

Keywords: norovirus, molecular motor biosensor, detection, F0F1-ATPase

Footnotes

These authors contributed equally to this work.

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