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. 2010 Feb 9;6(1):65–70. doi: 10.1007/s12519-010-0009-2

Human bocavirus in children with respiratory tract infection in Shanghai: a retrospective study

Mei Zeng 1,2,✉, Qi-Rong Zhu 1, Xiao-Hong Wang 1, Hui Yu 1, Jun Shen 1
PMCID: PMC7091469  PMID: 20143214

Abstract

Background

Human bocavirus (HBoV) was first reported in 2005. The worldwide presence of HBoV in children with acute respiratory tract infection (ARTI) has been confirmed. This study aimed to understand the prevalence and clinical features of HBoV in children with ARTI in Shanghai and explore the causative implication of HBoV in ARTI.

Methods

We retrospectively reviewed the medical records of 349 hospitalized children with ARTI between November 2006 and January 2007. From these children, 351 nasopharyngeal aspirate samples were collected; 325 of the samples were obtained from those with community-acquired ARTI and 26 from those with hospital-acquired ARTI. All samples were routinely screened for seven common respiratory viruses by immunofluorescence and further tested for HBoV by polymerase chain reaction.

Results

HBoV was detected in 16 (4.6%) of the 351 samples, and it was the second most commonly detected virus after respiratory syncytial virus. Three (19%) HBoV-positive samples were dual infection with respiratory syncytial virus or parainfluenza virus type 3. Of the 325 children with community-acquired ARTI, HBoV was identified to be positive in 11 (3.4%), of whom 6 were diagnosed with pneumonia with patchy or interstitial infiltrates in the lung indicated by chest radiography, 3 with bronchitis, and 2 with bronchial asthma exacerbation with attendant lung infection. Out of the 26 children with nosocomial ARTI, 5 (19.2%) had bronchitis which was found to be HBoV positive without co-detection of other viruses. The HBoV-positive children were aged 1.7 months to 43 months and their mean age was 13.7 months. Sixteen (100%) children had cough, 11 (68.8%) had wheezing, and 10 (62.5%) had fever.

Conclusions

HBoV was circulating in Shanghai during the study period, and which was detected frequently in children with ARTI. HBoV was found to be associated with community-acquired ARTI and may play a pathogenic role in nosocomial ARTI.

Key words: acute respiratory tract infection, children, community-acquired infection, hospital-acquired infection, human bocavirus

References

  • 1.Lopez A.D., Mathers C.D., Ezzati M., Jamison D.T., Murray C.J. Global and regional burden of disease and risk factors, 2001: systematic analysis of population health data. Lancet. 2006;367:1747–1757. doi: 10.1016/S0140-6736(06)68770-9. [DOI] [PubMed] [Google Scholar]
  • 2.Kesson A.M. Respiratory virus infections. Paediatr Respir Rev. 2007;8:240–248. doi: 10.1016/j.prrv.2007.07.003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Allander T., Tammi M.T., Eriksson M., Bjerkner A., Tiveljung-Lindell A., Andersson B. Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A. 2005;102:12891–12896. doi: 10.1073/pnas.0504666102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Sloots T.P., McErlean P., Speicher D.J., Arden K.E., Nissen M.D., Mackay I.M. Evidence of human coronavirus HKU1 and human bocavirus in Australian children. J Clin Virol. 2006;35:99–102. doi: 10.1016/j.jcv.2005.09.008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Ma X., Endo R., Ishiguro N., Ebihara T., Ishiko H., Ariga T., et al. Detection of human bocavirus in Japanese children with lower respiratory tract infections. J Clin Microbiol. 2006;44:1132–1134. doi: 10.1128/JCM.44.3.1132-1134.2006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Bastien N., Brandt K., Dust K., Ward D., Li Y. Human Bocavirus infection, Canada. Emerg Infect Dis. 2006;12:848–850. doi: 10.3201/eid1205.051424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Arnold J.C., Singh K.K., Spector S.A., Sawyer M.H. Human bocavirus: prevalence and clinical spectrum at a children’s hospital. Clin Infect Dis. 2006;43:283–288. doi: 10.1086/505399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Weissbrich B., Neske F., Schubert J., Tollmann F., Blath K., Blessing K., et al. Frequent detection of bocavirus DNA in German children with respiratory tract infections. BMC Infect Dis. 2006;6:109. doi: 10.1186/1471-2334-6-109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Choi E.H., Lee H.J., Kim S.J., Eun B.W., Kim N.H., Lee J.A., et al. The association of newly identified respiratory viruses with lower respiratory tract infections in Korean children, 2000–2005. Clin Infect Dis. 2006;43:585–592. doi: 10.1086/506350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Foulongne V., Olejnik Y., Perez V., Elaerts S., Rodière M., Segondy M. Human bocavirus in French children. Emerg Infect Dis. 2006;12:1251–1253. doi: 10.3201/eid1208.060213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Kesebir D., Vazquez M., Weibel C., Shapiro E.D., Ferguson D., Landry M.L., et al. Human bocavirus infection in young children in the United States: molecular epidemiological profile and clinical characteristics of a newly emerging respiratory virus. J Infect Dis. 2006;194:1276–1282. doi: 10.1086/508213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Manning A., Russell V., Eastick K., Leadbetter G.H., Hallam N., Templeton K., et al. Epidemiological profile and clinical associations of human bocavirus and other human parvoviruses. J Infect Dis. 2006;194:1283–1290. doi: 10.1086/508219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Kaplan N.M., Dove W., Abu-Zeid A.F., Shamoon H.E., Abd-Eldayem S.A., Hart C.A. Human bocavirus infection among children, Jordan. Emerg Infect Dis. 2006;12:1418–1420. doi: 10.3201/eid1209.060417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Smuts H., Hardie D. Human bocavirus in hospitalized children, South Africa. Emerg Infect Dis. 2006;12:1457–1458. doi: 10.3201/eid1209.051616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Regamey N., Frey U., Deffernez C., Latzin P., Kaiser L. Swiss Paediatric Respiratory Research Group. Isolation of human bocavirus from Swiss infants with respiratory infections. Pediatr Infect Dis J. 2007;26:177–179. doi: 10.1097/01.inf.0000250623.43107.bc. [DOI] [PubMed] [Google Scholar]
  • 16.Fry A.M., Lu X., Chittaganpitch M., Peret T., Fischer J., Dowell S.F., et al. Human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand. J Infect Dis. 2007;195:1038–1045. doi: 10.1086/512163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Qu X.W., Duan Z.J., Qi Z.Y., Xie Z.P., Gao H.C., Liu W.P., et al. Human bocavirus infection, People’s Republic of China. Emerg Infect Dis. 2007;13:165–168. doi: 10.3201/eid1301.060824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Naghipour M., Cuevas L.E., Bakhshinejad T., Dove W., Hart C.A. Human bocavirus in Iranian children with acute respiratory infections. J Med Virol. 2007;79:539–543. doi: 10.1002/jmv.20815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Maggi F., Andreoli E., Pifferi M., Meschi S., Rocchi J., Bendinelli M. Human bocavirus in Italian patients with respiratory diseases. J Clin Virol. 2007;38:321–325. doi: 10.1016/j.jcv.2007.01.008. [DOI] [PubMed] [Google Scholar]
  • 20.Lau S.K., Yip C.C., Que T.L., Lee R.A., Au-Yeung R.K., Zhou B., et al. Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong. J Infect Dis. 2007;196:986–993. doi: 10.1086/521310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Pozo F., García-García M.L., Calvo C., Cuesta I., Pérez-Breña P., Casas I. High incidence of human bocavirus infection in children in Spain. J Clin Virol. 2007;40:224–228. doi: 10.1016/j.jcv.2007.08.010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Christensen A., Nordbø S.A., Krokstad S., Rognlien A.G., Døllner H. Human bocavirus commonly involved in multiple viral airway infections. J Clin Virol. 2008;41:34–37. doi: 10.1016/j.jcv.2007.10.025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Allander T., Jartti T., Gupta S., Niesters H.G., Lehtinen P., Osterback R., et al. Human bocavirus and acute wheezing in children. Clin Infect Dis. 2007;44:904–910. doi: 10.1086/512196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24.Endo R., Ishiguro N., Kikuta H., Teramoto S., Shirkoohi R., Ma X., et al. Seroepidemiology of human bocavirus in Hokkaido prefecture, Japan. J Clin Microbiol. 2007;45:3218–3223. doi: 10.1128/JCM.02140-06. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Kahn J.S., Kesebir D., Cotmore S.F., D’Abramo A., Cosby C., Weibel C., et al. Seroepidemiology of human bocavirus defined using recombinant virus-like particles. J Infect Dis. 2008;198:41–50. doi: 10.1086/588674. [DOI] [PubMed] [Google Scholar]
  • 26.Lin F., Guan W., Cheng F., Yang N., Pintel D., Qiu J. ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV. J Virol Methods. 2008;149:110–117. doi: 10.1016/j.jviromet.2007.12.016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Kantola K., Hedman L., Allander T., Jartti T., Lehtinen P., Ruuskanen O., et al. Serodiagnosis of human bocavirus infection. Clin Infect Dis. 2008;46:540–546. doi: 10.1086/526532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Zeng M., Wang X.H., Yu H., Zhu Q.R. Epidemiology of common respiratory viruses among children with acute respiratory tract infections in Shanghai. Zhonghua Chuanranbing Zazhi. 2008;26:527–532. [Google Scholar]
  • 29.Subspecialty Group of Respiratory Diseases. Pediatric Society. Chinese Medical AssociationEditorial Board. Chinese Journal of Pediatrics; Chinese Medical Association. The routine for prevention and treatment of bronchial asthma in children (for trial) Zhonghua Er Ke Za Zhi. 2004;42:100–106. [PubMed] [Google Scholar]
  • 30.Bonzel L., Tenenbaum T., Schroten H., Schildgen O., Schweitzer-Krantz S., Adams O. Frequent detection of viral coinfection in children hospitalized with acute respiratory tract infection using a real-time polymerase chain reaction. Pediatr Infect Dis J. 2008;27:589–594. doi: 10.1097/INF.0b013e3181694fb9. [DOI] [PubMed] [Google Scholar]
  • 31.Catalano-Pons C., Bue M., Laude H., Cattan F., Moulin F., Menager C., et al. Human bocavirus infection in hospitalized children during winter. Pediatr Infect Dis J. 2007;26:959–960. doi: 10.1097/INF.0b013e3181256583. [DOI] [PubMed] [Google Scholar]
  • 32.Myint S. Recent advances in the rapid diagnosis of respiratory tract infection. Br Med Bull. 2002;61:97–114. doi: 10.1093/bmb/61.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 33.Völz S., Schildgen O., Klinkenberg D., Ditt V., Müller A., Tillmann R.L., et al. Prospective study of Human Bocavirus (HBoV) infection in a pediatric university hospital in Germany 2005/2006. J Clin Virol. 2007;40:229–235. doi: 10.1016/j.jcv.2007.07.017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Longtin J., Bastien M., Gilca R., Leblanc E., de Serres G., Bergeron M.G., et al. Human bocavirus infections in hospitalized children and adults. Emerg Infect Dis. 2008;14:217–221. doi: 10.3201/eid1402.070851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 35.Esposito S., Bosis S., Niesters H.G., Tremolati E., Sabatini C., Porta A., et al. Impact of human bocavirus on children and their families. J Clin Microbiol. 2008;46:1337–1342. doi: 10.1128/JCM.02160-07. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Bastien N., Chui N., Robinson J.L., Lee B.E., Dust K., Hart L., et al. Detection of human bocavirus in Canadian children in a 1-year study. J Clin Microbiol. 2007;45:610–613. doi: 10.1128/JCM.01044-06. [DOI] [PMC free article] [PubMed] [Google Scholar]

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