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. 2011 Oct 28;52(5):511. doi: 10.1007/s13580-011-0007-x

Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor

Suk-Min Ko 1, Hyeon-Jin Sun 1, Myung Jin Oh 2, In-Ja Song 1, Min-Jae Kim 1, Hyun-Sook Sin 1, Chang-Hyo Goh 3, Yong-Woo Kim 1, Pyung-Ok Lim 1,4, Hyo-Yeon Lee 1,5,✉, Suk Weon Kim 2,✉
PMCID: PMC7100749  PMID: 32226733

Abstract

Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.

Additional key words: aquatic plant, porcine diarrhea, spike protein, transformation, vaccine

Footnotes

These authors are contributed equally to this work.

Contributor Information

Hyo-Yeon Lee, Email: hyoyeon@cheju.ac.kr.

Suk Weon Kim, Email: kimsw@kribb.re.kr.

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