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. 1997;25(2):95–100. doi: 10.1007/BF02113584

Role of interleukin-8 in community-acquired pneumonia: Relation to microbial load and pulmonary function

Sabine Bohnet 1, U Kötschau 1, J Braun 1, K Dalhoff 1,✉
PMCID: PMC7101691  PMID: 9108184

Summary

In pneumonia local phagocyte activation is crucial for clearing of pathogenic microorganisms. In this context alveolar macrophage interleukin-8 secretion, phagocyte oxidative response and concentrations of lavage proteins were quantified, including interleukin-8, in 31 patients with pneumonia, 13 age matched patients with peripheral lung consolidation and six healthy volunteers; these findings were related to the impairment of gas exchange and the bacterial load in the alveolar space. Increased interleukin-8 levels were found in bronchoalveolar lavage fluid (BALF) and in alveolar macrophage supernatants from patients with pneumonia (214 ng/105 AM±121 vs 71 ng/105 AM±35 and 66 ng/105 AM±30, p<0.05). Interleukin-8 release from alveolar macrophages correlated with the upregulated spontaneous luminol enhanced oxidative response of pulmonary phagocytes but not with the neutrophil count in BALF. In pneumonia patients a significant difference was found between patients with 104 or more colony forming units (CFU)/ml BALF of one pathogen and patients with less CFU or nonspecific microbiological results (261 ng/105 AM±89 vs 179 ng/105 AM±81 and 7.5 ng/ml BALF±17 vs 0.44 ng/ml BALF±1, p<0.05). Further, a negative correlation between interleukin-8 release of alveolar macrophages and the arterial pO2 at the time of BALF could be demonstrated (r=−0.47, p<0.05). The results demonstrate local cellular activation in community-acquired pneumonia, which is related to the bacterial load in the alveolar space and to impairment of gas exchange. This is consistent with the hypothesis that pulmonary phagocytes play a central role in the pathogenesis of bacterial pneumonia, contributing not only to bacterial clearing but also to local tissue damage.

Keywords: Colony Form Unit, Alveolar Macrophage, Luminol, Bacterial Load, Bronchoalveolar Lavage Fluid

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