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. 2014 Jun 10;20(8):591–599. doi: 10.1007/s11655-014-1758-x

Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats

Lu-ping Tang 1, Wei Xiao 2, Yi-fang Li 1, Hai-bo Li 2, Zhen-zhong Wang 2, Xin-sheng Yao 1, Hiroshi Kurihara 1, Rong-rong He 1,✉
PMCID: PMC7101712  PMID: 24916807

Abstract

Objective

To evaluate the protective effects of Reduning Injection (热毒宁注射液, RDN), a patent Chinese medicine, on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats and its underlying mechanisms of action.

Methods

Sixty male Sprague-Dawley rats were randomly divided into 6 groups, including normal control, model, dexamethasone (DEX, 5 mg/kg), RDN-H (720 mg/kg), RDN-M (360 mg/kg) and RDN-L (180 mg/kg) groups, with 10 rats in each group. Rats were challenged with intravenous injection of LPS 1 h after intraperitoneal treatment with RDN or DEX. At 6 h after LPS challenge, lung tissues and bronchoalveolar lavage fluid (BALF) were collected, and the number of inflammatory cells was determined. The right lungs were collected for histopathologic examination, measurement of gene and protein expressions, superoxide dismutase (SOD) and myeloperoxidase (MPO) activities.

Results

In vivo pretreatment of RDN (360, 720 mg/kg) significantly reduced the weight of wet to dry (W/D) ratio of lung, protein content in BALF, and led to remarkable attenuation of LPS-induced histopathological changes in the lungs. Meanwhile, RDN enormously decreased BALF total inflammatory cells, especially neutrophil and macrophage cell numbers. Moreover, RDN increased SOD activity, inhibited MPO activity, alleviated LPS-induced tumor neurosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) expression in lung tissues. Furthermore, RDN (720 mg/kg) efficiently weakened nuclear factorkappa B (NF-κB) gene and protein expression.

Conclusion

Anti-inflammatory effects of RDN was demonstrated to be preventing pulmonary neutrophil infiltration, lowering MPO activity, TNF-α and iNOS gene expression by inhibiting NF-κB activity in LPS-induced ALI.

Keywords: acute lung injury, lipopolysaccharide, neutrophils, chemokine, nuclear factor-kappa B, Reduning Injection, Chinese patent medicine

Footnotes

Supported by Distinguished Trans-Century Training Program Foundation for the Talents of the State Education Commission, China (No. NCET-12-0678), Natural Science Foundation of Guangdong Province, China (No. S20120011316), Science and Technology Program of Guangzhou, China (No. 2012J22000073), Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (No. LYM11023)

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