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. 1993;17(2):199–204. doi: 10.1007/BF00916105

Increased serum catalase activity in rats subjected to thermal skin injury

Jonathan A Leff 1, Lisa K Burton 1, Elaine M Berger 1, Benjamin O Anderson 1, Christian P Wilke 1, John E Repine 1
PMCID: PMC7102124  PMID: 8491514

Abstract

We found that: rats subjected to thermal skin injury (burn) had increased serum hydrogen peroxide (H2O2) scavenging activity, serum catalase activity, erythrocyte (RBC) fragility, and edematous lung injury (lung leak) when compared to sham-treated rats. Serum H2O2 scavenging activity was inhibited by addition of sodium azide, a catalase inhibitor. Treatment of rats with the oxygen radical scavenger, dimethylthiourea (DMTU), decreased RBC fragility and lung leak but did not alter increased H2O2 scavenging or catalase activity of serum from rats subjected to skin burn. We conclude that increased serum catalase activity is a consequence of thermal skin injury and that increased serum catalase activity may be a mechanism that modulates H2O2-dependcnt processes following skin burn.

Keywords: Catalase, Azide, Lung Injury, Scavenge Activity, Catalase Activity

Footnotes

All work was done during Dr. Leff's tenure of a Clinician-Scientist Award from the American Heart Association and supported by grants from the National Institutes of Health (PO1-AM-35098, BRS 983), Colorado Heart Association, American Lung Association, Ronald McDonald Children's Charities, Johnson & Johnson, Williams Family Foundation, Council for Tobacco Research Inc., Swan, Hill, Kleberg, Sachs, Etonner and American Express Foundations.

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