Fig. 8.

Downregulation of PALM3 suppresses LPS-induced NF-κB and IRF3 activation in lungs. Rat lung tissues were collected and the NF-κB-DNA-binding activity (a) was measured by ELISA. Data are expressed as means ± SEM (n = 6). *P < 0.05 vs. Ad.V group at each time point and #P < 0.05 vs. 0 h time point. b Detection of NF-κB phosphor-p65 protein levels in lung tissues at 24 h after LPS-stimulation by western blot. Upper panel of b is a representative photograph. Normal and ALI rats were used as control groups. c Detection of the phospho-IRF-3 protein levels in lung tissues at 24 h after LPS-stimulation by western blot. Upper panel of c is a representative photograph. Normal and ALI rats were used as control groups. Data are expressed as means ± SEM (n = 6). *P < 0.05 vs. LPS-treated and LPS + Ad.V-treated rats and #P < 0.05 vs. normal rats.