Fig. 2.

Removal of PB sequences from biPSCs. (A) The map of plasmid vector pMax-GFP. (B) Two optimal transfection programs U013 and T016 of biPSCs in Lonza nucleofection system were selected by GFP fluorescence intensity after electroporation. (C) Morphology of the pMax-GFP transfected biPSC colonies after 4 days' culture. (D) Southern blot analysis of the biPSC lines obtained from transfecting biPSCs with pCAG-PBase vector and subsequent 5 days FIAU screening (left), and PCR validating of the seemingly “transgene-free” clones from Southern blot analysis (right), the serial number labeled is consistent with that of Southern blot analysis. biPSC, bat-induced pluripotent stem cell; GFP, green fluorescent protein; FIAU, Fialuridine; PCR, polymerase chain reaction (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).