Primers used in this study.
| PCR target region | Sequences (5′–3′) | Applications |
|---|---|---|
| pSTEM-h103 PB probe | F: TGACGTACGTTAAAGATAATCATGC | Southern blot (340 bp) |
| R: GCTTCGATTAATAAGTATAATTTGTTTCT | ||
| pSTEM-h103 Neo-IRES | F: GGACAGGTCGGTCTTGACAAAAAG | GoTaq PCR (569 bp) |
| R: TCTGTTGAATGTCGTGAAGGAAGC | ||
| b-βactin | F: ACTGGGACGACATGGAGAAG | Real-time PCR as reference genes |
| R: AGAGGCGTACAGGGACAGC | ||
| bOct4 | F: GGTACACCCAGGCCGATGT | Real-time PCR for pluripotent genes expression |
| R: GATGGTCGTTTGGCTGAACA | ||
| bSox2 | F: CTGCGAGCGCTGCACAT | |
| R: TCATGAGCGTCTTGGTTTTCC | ||
| bKlf4 | F: CGAACCCACACAGGTGAGAAA | |
| R: CTGAGCGGGCAAACTTCCA | ||
| bcMyc | F: ACGTCAGCTTCGCCAACAG | |
| R: GTTCTCTTCCTCGTCGCAGAA | ||
| Bat genomic Oct4 | F: ATACTTCCTCAAGGCTCCCAGGAC | GoTaq PCR (281 bp) |
| R: GGGTGCATTTCCCACGTAAAGATA | ||
| Bat genomic Hprt | F: TACTGCAGGAGGTCAGCGCTACTAA | GoTaq PCR (262 bp) |
| R: ATCACAACACTGGGGCTGTGAGTC |