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. 2007 Jun 22;367(1):41–50. doi: 10.1016/j.virol.2007.05.013

Fig. 3.

Fig. 3

Analysis of ATPase activities of the wild-type and mutant helicase-like domains of BaMV replicase by TLC. The activity assay was carried out for 20 min in 10 μl solution that contained basically 20 μM ATP, 5 μCi [α-32P] ATP (6000 Ci/mmol, PerkinElmer) and 1 pmol purified protein. Presence or absence of 0.16 μM RNA (200 nt) was as indicated. The reaction products were analyzed by PEI TLC and autoradiography.