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. 2020 Feb 1;7(1-2):1–9. doi: 10.18632/oncoscience.496

Figure 4. Overexpressed miR-145 increases LC3 I & II protein levels in neuroblastoma cells.

Figure 4

(A) LC3 protein was detected with immunoblotting in cells. β-actin served as a loading control. (B) Parental, CDDP-R, Vin-R, and Rad-R BE(2)-C cells transfected with miR-CON and miR-145 plasmid. LC3 I & II protein level was detected. β-actin served as a protein loading control. (C) Parental, CDDP-R, Vin-R, and Rad-R BE(2)-C cells co-transfected with miR-CON or miR-145 plasmid with eGFP-LC3 plasmid, treated with or without BafA1 (200 nM) for 24 h and then assessed for autophagy after 48 h transfection. Representative fluorescence confocal microscopic images were taken. (D) Cells with GFP-LC3 puncta were quantified as percentage of total GFP cells for each experiment (n = 3). At least 20 cells were counted in each individual experiment. Values shown are mean ± SEM of three separate experiments (* P< 0.05 vs. miR-CON).