Figure 3.

Establishment and characterization of T_H1 cell induced lung inflammation. HA-specific CD4⁺ T cells from the spleens of TCR-HA transgenic mice were sorted by flow cytometry and differentiated into T_H1 cells in vitro, success was validated by intracellular staining of IFN-γ on day 6 (A). Exemplary dot plots are shown. 5 × 10⁵ HA-specific T_H1 cells were adoptively transferred into SPC-HA transgenic mice and non-transgenic littermates. Severity of inflammation was assessed by weight loss (B) and histology on day 6 (C). Expression of pro-inflammatory cytokines and chemokines was assessed by real-time PCR (D) and Luminex analysis (E). Statistical analysis was performed using Student's t-test (*p < 0.05; **p < 0.01; ***p < 0.001; n.d., not detectable).