Figure 6.
A series of optical images for magnetic MnFe2O4 nanoparticle complexes with different concentration of the influenza A virus. (A) 1 × PBS as negative control (NC), (B) 0.007, (C) 0.03, (D) 0.125, (E) 0.50, (F) 2.0 and (G) 8.0 HAU. (H) The comparison of optical signals between M450 beads and nanoparticles, from NC to 8.0 HAU: the measurements of nanoparticle tests were 440 ± 39, 557 ± 88, 860 ± 135, 1449 ± 183, 2032 ± 279, 2500 and 2500 mV, and the measurements of M450 were 368 ± 15, 390 ± 22, 462 ± 29, 531 ± 37, 774 ± 52, 1199 ± 155 and 2198 ± 233 mV. (I) A series of fluorescence images for nanoparticle complexes with clinical specimens. (J) Optical intensity histogram of pre-clinical tests from nanoparticles was measured to be 1225 ± 191, 1140 ± 212, 2295 ± 120, 2500 and 1964 ± 258 mV (n = 3), with one-way ANOVA analysis, F(1,3) = 27.52 > 10.13, P = 0.01 < 0.05, showing that negative and positive specimens were significantly different. *Note that the high-concentration influenza viral samples, (H) 2.0 and 8.0 HAU and (J) specimen 4 exhibit optical signals that are higher than the maximum value of the optical detection system (2500 mV).