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. 2020 Mar 16;23(4):100987. doi: 10.1016/j.isci.2020.100987

Figure 1.

Figure 1

Identification of a VHH Specific for Monocyte-Derived Dendritic Cells

(A) Strategy for identifying novel surface markers for ascites dendritic cells (DCs).

(B) Ascites cells were stained with VHH D4. Stainings for DC and macrophages (Macro) are shown (representative of five individual donors). Gray shaded histograms are fluorescence-minus-one controls.

(C) Monocytes were cultured with M-CSF, IL-4, and TNFa to generate DC (mo-DC) and macrophages (mo-Mac) or with GM-CSF and IL-4 to generate DC (GM + IL4 mo-DC). Cells were stained with VHH D4. Gray shaded histograms are fluorescence-minus-one controls. Representative of four individual donors.