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. 2019 Aug 1;105(4):999–1012. doi: 10.3324/haematol.2018.207001

Figure 4.

Figure 4.

Effect of ectopic expression of CD99 long isoform on reactive oxygen species (ROS) levels, DNA damage and cell apoptosis. (A and B) ROS levels of EV, CD99-L and CD99-S cells determine by flow cytometry using the cell ROX reagent in THP-1 cells (n=4) measured by flow cytometry. (C) Western blot analysis of H2Axγ in THP-1 EV, CD99-L and CD99-S. (D and E) Apoptosis analysis of EV, CD99-L and CD99-S cells determined by annexin V+ cells through flow cytometry in THP-1, U937 and MOLM-13 cells (n=4). (F) Western blot analysis of cleaved caspase-3 in THP-1 EV, CD99-L, and CD99-S. (**** P<0.0001; ** P<0.01).