Table 2.
Details of all dogs positive for Clostridium perfringens cpe+ or toxigenic Clostridium difficile.
| ID | Age (months) | Feacal characteristic |
Clostridium difficile |
Clostridium perfringens |
Other enteropathogens | Outcome | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| A/B toxin | Isolation | Ribotypea | cpe | netE/F | netG | CPE | |||||
| 1 | 48 | Mushy | – | – | – | + | – | – | + | – | Recovered. |
| 2 | 3 | Bloody | – | – | – | + | – | – | + | Coronavirus | Died |
| 3 | 125 | Bloody | – | – | + | – | – | – | – | Recovered. | |
| 4 | 61 | Bloody | – | – | – | + | + | – | – | Giardia sp. | Recovered. |
| 5 | 18 | Bloody | – | – | – | + | + | + | + | – | Recovered. |
| 6 | 133 | Bloody | – | – | – | + | + | – | + | – | Recovered. |
| 7 | 18 | Bloody | – | – | – | + | + | – | – | – | Recovered |
| 8 | 19 | Bloody | + | A+B+CDT- | 014/020 | + | + | – | – | – | Recovered. |
| 9 | 145 | Bloody | + | A+B+CDT- | 014/020 | + | + | + | – | – | Died. |
| 10 | 36 | Bloody | + | A+B+CDT- | 106 | + | + | + | + | EPEC (eae+) | Died. |
| 11 | 19 | Bloody | + | A + B + CDT- | 014/020 | – | – | – | – | – | Recovered |
| 12 | 181 | Bloody | + | A + B + CDT- | BR1 | – | – | – | – | CPV-2b and Giardia sp. | Recovered |
| 13 | 6 | Bloody | + | A + B + CDT- | BR1 | – | – | – | – | – | Died |
| 14 | 168 | Mushy | + | A + B + CDT- | 014/020 | – | – | – | – | – | Recovered |
| 15 | 48 | Bloody | + | A + B + CDT- | 106 | – | – | – | – | – | Died |
| 16 | 121 | Bloody | – | A + B + CDT- | BR2 | – | – | – | – | – | Died |
| 17 | 5 | Bloody | – | A + B + CDT- | BR3 | – | – | – | – | – | Died |
| 18 | 23 | Mushy | – | A + B + CDT- | BR4 | – | – | - | - | Giardia sp. | Recovered. |
| 19 | 120 | Mushy | – | A + B + CDT- | 602 | – | – | – | – | – | Recovered. |
Legend: EPEC – Enteropathogenic E. coli; CPV-2b – Canine parvovirus type 2b; CPE – C. perfringens enterotoxin; A – Toxin A encoding gene (tcdA); B – Toxin B encoding gene (tcdB); CDT – binary toxin gene (cdtB).
PCR ribotypes for which the reference strains were available are designated by international Cardiff nomenclature, while others are designated by internal nomenclature (BRA and number).