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. 2017 Jul 12;510:67–75. doi: 10.1016/j.virol.2017.07.008

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© 2017 Elsevier Inc.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 5 — JEV protein E physically interacts with BST2. 293T cells were cotransfected with pBST2 and plasmid expressing E-flag, ME-flag or M-HA. At 48 h post transfection, cell lysates were analyzed by co-IP. Co-IP was pulled down using the anti-BST2, anti-flag or anti-HA antibody. Proteins were immunoprecipitated with the anti-flag (A) or anti-BST2 antibody (B) as indicated. (C) Proteins were immunoprecipitated with the anti-HA or anti-BST2 antibody as indicated. (D) HeLa cells were infected with JEV at a MOI of 25. At 48 h post infection, cell lysates were analyzed by co-IP. Co-IP was pulled down using the anti-BST2 antibody. One representative experiment out of three is shown. (E) Colocalization of BST2 with JEV E-flag or ME-flag. 293T cells cotransfected with pBST2 and plasmid expressing E-flag or ME-flag were costained with anti-flag (red) and anti-BST2 (green) antibodies. Nuclei were counterstained with DAPI (blue). Representative confocal images from three independent experiments are shown. Scale bars in all panels represent 10 µm. (F) HeLa cells were transfected with plasmid expressing protein E. The surface expression of BST2 was analyzed by flow cytometry.