Fig. 8.

Chemical inhibition of ERK1/2 activation impairs viral protein translation. Inhibitor-treated Vero cells were mock-infected or infected with PEDV (MOI of 1) for 1 h and were further cultivated in the presence or absence of each inhibitor. At 48 hpi, cellular lysates were collected, resolved by SDS-PAGE, transferred to a nitrocellulose membrane, and immunoblotted by using the antibody against the PEDV N protein. The blot was also reacted with mouse MAb against β-actin to verify equal protein loading. Each viral protein expression was quantitatively analyzed by densitometry in terms of the relative density value to the β-actin gene and inhibitor-treated sample results were compared to DMSO-control results. Values shown are representative of the mean from three independent experiments and error bars denote standard deviations. *, P=0.001 to 0.05; †, P<0.001.