Fig. 1.

rcMBL inhibits IBV-Beaudette infection of BHK 21 cells in a concentration dependent manner. BHK-21 cells were inoculated after pre-incubation of IBV-Beaudette (MOI of 0.1) with various concentrations of rcMBL. A) Infection was determined by immunofluorescence using an antibody directed against the IBV nucleocapsid protein at 8 h post-infection. Bar: 50 µm. B) Infection was determined by quantitative PCR: the IBV viral genome expression relative to GAPDH gene expression was determined and expressed relative to the no-rcMBL control. C) The toxic effects of rcMBL on BHK-21 cells was determined by the WST-1 assay. Asterisks indicate statistical significant difference compared to the no rcMBL control. Shown are mean ± SEM of three independent experiments.