Fig. 3.

Calcium-dependent binding of rcMBL to IBV M41 and S1 spike protein. ELISA of rcMBL binding to A) IBV M41, and B) recombinant M41 S1 protein. Shown are mean ± SEM of three independent experiments. Circles: binding in the presence of 5 mM Ca²⁺. Squares: binding in the presence of 5 mM Ca²⁺ and 100 μg mannan. Triangles: binding in the presence of 5 mM Ca²⁺ and 10 mM EDTA.