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Multiple sequence alignment of the partial S1 spike protein. The sequence above shows amino acid positions 35–70. Previous studies have shown there are four amino acid positions (38, 43, 63, and 69) that are important for binding of the spike on tracheal tissue (Becker et al., 1967, Promkuntod et al., 2014). Multiple sequence alignment was performed using ClustalW and eBioX software.
