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Recognition of Stx2B and Stx2 holotoxin by IgY and IgG antibodies using Western immunoblotting. Lanes 1 and 3. Purified recombinant Stx2B protein. Lanes 2 and 4. Stx2 holotoxin. Lane M. prestained protein standard. Membrane was incubated with purified anti-Stx2B IgY and anti-IgY POD (A) or rabbit sera and anti-IgG POD (B).Arrow: Stx2B subunit position.
