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. 2011 Jul 22;58(4):380–388. doi: 10.1016/j.toxicon.2011.07.009

Fig. 5.

Fig. 5

Detection of antibody binding to Stx2 holotoxin in solution by a sandwich ELISA. Plates were coated with purified IgY or IgG antibodies, followed by incubation with 3-fold serial dilutions of Stx2 supernatant. Anti-Stx2B IgG POD was used as the second antibody. DH5α supernatant was used as negative control.