Fig. 2.

Characterization of BMV RdRp in vitro copying products of mixtures of RW(−) RNA3 and sgRNA3a templates. Template RNA mixtures were copied as described in Materials and methods, and the radioactive RdRp products were separated in a 1% denaturing agarose gel. The sgRNA3a variants are specified above (lanes 3 to 5). Lanes 1 and 2 contain copying products of RW(−) RNA3 alone [symbolized by (−)] with half the amount loaded in lane 1. Lane 6 contains a mixture of three radioactive in vitro transcripts as migration standards (the RNA2 marker was synthesized from plasmid pB2TP5 after linearization with MluI).