Table 1.
PCR primers used in conventional and real time PCR techniques.
| Primer name | Sequence (5'–3') | Fragment size (Bps) | Reference |
|---|---|---|---|
| IBV N | F- GACGGAGGACCTGATGGTAAR- CCCTTCTTCTGCTGATCCTG | 206 | This study |
| IFN-α | F- ATCCTGCTGCTCACGCTCCTTCTR- GGTGTTGCTGGTGTCCAGGATG | 198 | (Villanueva et al., 2011) |
| IFN-β | F- AGCAAGGACAAGAAGCAAGCR- CGTGCCTTGGTTTACGAAGC | 177 | (Esnault et al., 2011) |
| IFN-γ | F- ACACTGACAAGTCAAAGCCGCACA R- AGTCGTTCATCGGGACCTTGGC | 129 | (Villanueva et al., 2011) |
| TLR3 | F- TCAGTACATTTGTAACACCCCGCCR- GGCGTCATAATCAAACACTCC | 256 | (Villanueva et al., 2011) |
| TLR7 | F- TTCTGGCCACAGATGTGACCR- CCTTCAACTTGGCAGTGCAG | 219 | (Villanueva et al., 2011) |
| IL-1β | F- GTGAGGCTCAACATTGCGCTGTAR- TGTCCAGGCGGTAGAAGATGAAG | 214 | (Villanueva et al., 2011) |
| iNOS | F- GGCAGCAGCGTCTCTATGACTTGR- GACTTTAGGCTGCCCAGGTTG | 185 | (St Paul et al., 2013) |
| MyD88 | F- AGCGTGGAGGAGGACTGCAAGAAG R- CCGATCAAACACACACAGCTTCAG | 264 | (Villanueva et al., 2011) |
| TRIF | F- GCTCACCAAGAACTTCCTGTGR- AGAGTTCTCATCCAAGGCCAC | 181 | (Villanueva et al., 2011) |
| β-actin | F- CAACACAGTGCTGTCTGGTGGTAR- ATCGTACTCCTGCTTGCTGATCC | 205 | (Villanueva et al., 2011) |
| Ubiquitin | F- GGGATGCAGATCTTCGTGAAAR- CTTGCCAGCAAAGATCAACCTT | 147 | (De Boever et al., 2008) |