Fig. 2.

PDCoV and TGEV infection of PAMs and lung-derived fibroblast-like cells. (A) ANPEP WT and KO PAMs were infected with different MOIs of TGEV or PDCoV. After 24 h from infection, the cells were stained and fixed with AlexaFluor488-labeled anti-TGEV or anti-PDCoV antibodies and the percent antigen-positive cells recorded. (B) ANPEP WT and KO lung-derived fibroblast-like cells were infected with TGEV or PDCoV at an MOI of 0.0001–10. After 24 h from infection, the cells were stained and fixed with AlexaFluor488-labeled anti-TGEV or anti-PDCoV antibodies and the percent antigen-positive cells determined. Results are shown as the mean and standard deviation of results from three ANPEP WT and three KO pigs.