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. 2007 Jul 4;271(1):21–30. doi: 10.1016/j.aquaculture.2007.06.029

Fig. 2.

Fig. 2

(A) Expression of target genes of each specific siRNA group was examined following siRNA injection and virus challenge. Virus group was infected WSSV without siRNA injection. Specific siRNA group was injected specific siRNAs of selected WSSV genes and WSSV at the same time. β-actin of L. vannamei was selected for inner-control. (B) Expression of each selected gene of mutant siRNA group was examined after siRNA injection and virus challenge. Virus group was infected WSSV without siRNA injection. Mutant siRNA (siMUT) group was injected siMUTs and WSSV at the same time. (C) The relative quantity of each target gene expression was analyzed by the AlphaEase FC imaging system. X-axis showed different groups at 24 h, 36 h and 48 h test and there were 3 shrimp in each time. v was positive group, m was siMUT group, and s was specific siRNA group. Y-axis showed the ratio of electrophoresis band intensity of each target gene to that of β-actin. Each group included five ratios, they were dnapol/β-actin (rectangles with transverse lines), rr2/β-actin (diagonal rectangles), tk-tmk/β-actin (rectangles with vertical stripes), vp24/β-actin (filled rectangles) and vp28/β-actin (rectangles with gridding), respectively.