Fig. 5.

Protection conferred by rTGEVs expressing PRRSV antigens. (A) Percentage of animals showing respiratory symptoms. Observations were made daily during a time frame of 28 days post-challenge. ^⁎⁎, p-value<0.05, ^⁎, p-value<0.1. (B) Lung samples collected at 28 days post-challenge were stained with hematoxylin-eosin. Representative pictures obtained with a 10x objective are shown (left panels). Lung damage was scored by observation of 50 random fields per animal (right panel). (C) IL-8 levels in serum. Serum samples were collected at the indicated times post-immunization, mixed in pools of three animals per sample, and cytokine levels were analyzed by a 7-plex fluorescent microsphere immunoassay. Dots represent individual samples and solid lines represent mean titer for each group. ^⁎, p-value<0.1.