Fig. 6.

Changes in proinflammatory cytokines IL-6, IL-12, and TNF-α, and IFN-α genes mRNA expression induced by porcine epidemic diarrhea virus (PEDV) non S-INDEL and PEDV S-INDEL strains in intestinal mucosa (A-D). Ten pigs in each group were infected with PEDV non S-INDEL, PEDV S-INDEL or media (negative control) and five pigs from each group were necropsied at 3 and 7 days post-infection (dpi). The mRNA levels of IL-6 (A), IL-12 (B), TNF-α (C), and IFN-α (D) at the intestinal mucosa was determined individually in each animal by SYBR-green qRT-PCR. All samples were tested in triplicate and the results are expressed as fold changes relative to the control animals Data are presented as means ± standard errors. Significant difference between PEDV S-INDEL PEDV non S-INDEL and control group are expressed with their p values. *p < 0.05; **p < 0.01, ***p < 0.001.