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BDV Hu-H1 infection modifies the histone lysine acetylation (Kac) profile. After OL/Hu-H1 cells and control cells were harvested, the core histones from whole cell lysates were extracted. Extracted histones (2 μg) were subject to SDS-PAGE followed by Western blotting analysis to examine histone site-specific Kac in (A) H2A, (B) H2B, (C) H3, and (D) H4 through histone site-specific Kac antibodies.
