Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Neisseria meningitidis) | clpX | UniProtKB - Q9JYY3 | ||
| Gene (Neisseria meningitidis) | clpP | UniProtKB - Q9JZ38 | ||
| Strain, strain background (Escherichia coli) | BL21(DE3) | Sigma-Aldrich | CMC0016 | Chemically competent cells |
| Recombinant DNA reagent | pET28-NmClpP | Synthetic (GenScript) |
UniProtKB - Q9JYY3 | Plasmid containing ClpP |
| Recombinant DNA reagent | pET28a-NmClpX | Synthetic (GenScript) |
UniProtKB - Q9JZ38 | Plasmid containing ClpX |
| Recombinant DNA reagent | pET28a- GFP-SsrA |
(Ripstein et al., 2017) | Plasmid containing GFP-SsrA | |
| Chemical compound, drug | PKM-AMC | GenScript | Fluorogenic peptide for protease assays | |
| Software, algorithm | EPU | Thermo Fischer Scientific | EM imaging software | |
| Software, algorithm | cryoSPARC v2 | (Punjani et al., 2017) | RRID:SCR_016501 | EM reconstruction software |
| Software, algorithm | UCSF Chimera | (Pettersen et al., 2004) | RRID:SCR_004097 | Molecular Visualization Software |
| Software, algorithm | UCSF ChimeraX | (Goddard et al., 2018) | RRID:SCR_015872 | Molecular Visualization Software |
| Software, algorithm | Coot | (Emsley and Cowtan, 2004) | RRID:SCR_014222 | Protein Model Building Software |
| Software, algorithm | Phyre2 | (Kelley et al., 2015) | RRID:SCR_010270 | Protein Model Building Software |
| Software, algorithm | Rosetta | (Wang et al., 2015) | RRID:SCR_015701 | Protein Model Building Software |
| Software, algorithm | Phenix | (Adams et al., 2010) | RRID:SCR_014224 | Protein Model Building Software |
| Software, algorithm | Molprobity | (Arendall III et al., 2010) | RRID:SCR_014226 | Protein Model Evaluation Software |
| Software, algorithm | EMRinger | (Barad et al., 2015) | Protein Model Evaluation Software |