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. 2020 Mar 18;9:e52779. doi: 10.7554/eLife.52779

Figure 3. Loss of Piezo1 or both Piezo1/2 in embryonic limb mesenchyme led to reduced cartilage growth and osteoblast differentiation.

(a) Immunofluorescent images of Sox9 and Sp7 expression in the humerus sections of E16.5 embryos. (b) Representative BrdU of humerus sections of E16.5 embryos. The percentage of BrdU+ cells in the growth plate was quantified (n = 3, means ± SD). *p<0.05, **p<0.01, ***p<0.001, one-way ANOVA followed by Tukey’s multiple comparisons tests. Proliferative cartilage regions were indicated by double headed arrows (Figure 3—source data 1). (c) Representative TUNEL staining of humerus sections of E16.5 embryos. TUNEL signals in the muscle were indicated by arrows. (d) von Kossa staining and (e) Spp1 immunostaining of humerus sections from P0 littermate pups. Reduced staining was indicated by arrows. All scale bars, 100 μm. DAPI (blue) stain the nucleus.

Figure 3—source data 1. Quantification of cell proliferation.

Figure 3.

Figure 3—figure supplement 1. Immunostaining of Sox9, Col10 and Sp7 in sections of humerus cartilage from P0 pups.

Figure 3—figure supplement 1.

All scale bars: 100 μm. DAPI stains the nucleus.