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Expression of rTGEV-S from LcS transformants. Supernatants from overnight cultures of LcS-rTGEV-S (lane 3), LcS-pLP500 (lane 4) or LcS (lane 5) were separated by SDS–PAGE 10% and transferred electrophoretically to nitrocellulose before being incubated with convalescent swine anti-TGEV serum at a dilution of 1:1000. Bound antibodies were detected with HRP-conjugated anti-swine IgG antibodies. Lane 1: purified recombinant TGEV S protein; lane 2: MRS broth.
