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. 2009 Jan 30;27(11):1758–1763. doi: 10.1016/j.vaccine.2009.01.021

Fig. 1.

Fig. 1

Analysis the recombinant SARS-CoV N protein expression in vitro by Western blot. The expression of SARS-CoV N protein was determined in 293 cells transfected with pcDNA-N, or pcDNA3.1(+) by Western blot analysis. Rat anti-N-specific antibody was used at a 1:100 dilution for the detection of N protein expression. Lane 1: lysate of 293T cells transfected with pcDNA-N; Lane 2: lysate of 293T cells transfected with pcDNA3.1(+); Lane 3: Protein molecular weight marker (Gibco).