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Detection of WNV antigen in Vero cells by indirect immunofluorescence. Vero cells, uninfected (a), transfected with WNVsyn-transcribed RNA (b), infected with WNV wild-type virus (c), or infected with a WNVsyn virus stock (d) were tested for the expression of WNV proteins by indirect immunofluorescence two days after infections or transfection.
