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. 2010 Mar 6;28(19):3318–3324. doi: 10.1016/j.vaccine.2010.02.092

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Copyright © 2010 Elsevier Ltd. All rights reserved.

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Fig. 3 — Plaque morphology of wild-type WNV (a) and WNVsyn (b) at day 4 postinfection of Vero cells seeded in six-well plates with a titer of 100 TCID₅₀. Staining was carried out with neutral red for 4 h. Growth of synthetic (solid diamonds) and wild-type WNV (open squares) was analyzed in Vero (c) and C6/36 cells (d) infected with a MOI of 0.0001. The growth curve experiment was repeated once. The titers are means of two determinations ± SD indicated by the error bars.