Figure 3. . Leishmania donovani DNA extracted from purified amastigotes and determined by PCR targeting the kinetoplast sequence.

(A) 1 μl of eluate from each manufacturer's column was used as a template. (B) 10 μl of eluate from each manufacturer's column was used as a template. Eluates from three separate columns for each manufacturer were used.

M: 100-bp marker; NTC: No template control; POS: Positive control.