Fig. 3.

Comparison of mRNA expression profiles of PLA2s in normal and infection bronchitis tissues. a: ChPLA2-IB, b: ChPLA2-IIA, c: ChPLA2-IIE, d: ChPLA2-V and e: ChPLA2-X. Gene-specific primers were used to determine the expression profiles of the avian PLA2. Each chicken gene was analyzed twice independently using the Light Cycler 480 system. Error bars represent the observed standard deviation. The relative expression values were scaled to a range of 0–100. The values shown were calculated using β-actin as the housekeeping gene. Results are expressed as the mean +/− SEM percentage of the control value (n _ 3).∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.